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Cattle bHB(Beta-Hydroxybutyric Acid) ELISA Kit
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ELK8618
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48 T $265.00
96 T $376.00
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Overview

Product name: Cattle bHB(Beta-Hydroxybutyric Acid) ELISA Kit
Reactivity: Cattle
Alternative Names: OHB; Beta-Hydroxybutyrate; 3-Hydroxybutyric Acid
Assay Type: Competitive Inhibition
Sensitivity: 0.46 µg/mL
Standard: 100 µg/mL
Detection Range: 1.57-100 µg/mL
Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length: 2h
Research Area: Signal transduction;Metabolic pathway;Endocrinology;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Cattle bHB. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle bHB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle bHB in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (µg/mL) OD Corrected OD
100.00 0.195
50.00 0.386
25.00 0.527
12.50 0.823
6.25 1.168
3.13 1.589
1.57 1.796
0.00 2.213

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant bHB and the recovery rates were calculated by comparing the measured value to the expected amount of bHB in samples.
Matrix Recovery range Average
serum(n=5) 84-97% 90%
EDTA plasma(n=5) 95-107% 101%
Heparin plasma(n=5) 77-95% 93%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of bHB and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 88-103% 86-97% 92-106% 85-97%
EDTA plasma(n=5) 95-104% 82-96% 92-103% 90-99%
Heparin plasma(n=5) 92-101% 87-96% 85-96% 85-95%