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Human CA 27-29(cancer antigen 27-29) Microsample ELISA Kit
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ELK8602MS
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48 T $320.00
96 T $458.00
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Overview

Product name: Human CA 27-29(cancer antigen 27-29) Microsample ELISA Kit
Reactivity: Human
Alternative Names: CA 27-29
Assay Type: Sandwich
Sensitivity: 0.8 U/mL
Standard: 100 U/mL
Detection Range: 1.57-100 U/mL
Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Assay Length: 3.5h
Research Area: Cancer
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CA 27-29. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CA 27-29. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CA 27-29, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CA 27-29 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (U/mL) OD Corrected OD
100.00 2.110 2.033
50.00 1.512 1.435
25.00 1.212 1.135
12.50 0.922 0.845
6.25 0.571 0.494
3.13 0.267 0.190
1.56 0.212 0.135
0.00 0.077 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant CA 27-29 and the recovery rates were calculated by comparing the measured value to the expected amount of CA 27-29 in samples.
Matrix Recovery range Average
serum(n=5) 89-119% 104%
EDTA plasma(n=5) 86-123% 105%
Heparin plasma(n=5) 93-104% 99%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CA 27-29 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 84-113% 88-118% 83-95% 92-109%
EDTA plasma(n=5) 85-101% 82-92% 88-101% 88-115%
Heparin plasma(n=5) 90-102% 86-113% 92-117% 90-115%

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