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Rat TPO-Ab(anti-Thyroid-Peroxidase antibody) ELISA Kit
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ELK8352
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48 T $320.00
96 T $458.00
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Overview

Product name: Rat TPO-Ab(anti-Thyroid-Peroxidase antibody) ELISA Kit
Reactivity: Rat
Alternative Names: TPO Ab; TPO-Ab
Assay Type: Competitive Inhibition
Sensitivity: 1.25 IU/mL
Standard: 80 IU/mL
Detection Range: 1.25-80 IU/mL
Sample type: serum, plasma, cell lysates, cell culture supernates and other biological fluids
Assay length: 2h
Research Area: Signal transduction;Metabolic pathway;Infection immunity;Hormone metabolism;Dermatology;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat TPO-Ab. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat TPO-Ab. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat TPO-Ab in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (IU/mL) OD Corrected OD
80.00 0.191
40.00 0.379
20.00 0.567
10.00 0.959
5.00 1.152
2.50 1.511
1.25 1.835
0.00 2.123

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant TPO-Ab and the recovery rates were calculated by comparing the measured value to the expected amount of TPO-Ab in samples.
Matrix Recovery range Average
serum(n=5) 83-95% 89%
EDTA plasma(n=5) 87-99% 93%
Heparin plasma(n=5) 92-105% 98%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TPO-Ab and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 87-103% 97-105% 79-90% 87-96%
EDTA plasma(n=5) 87-98% 87-100% 85-94% 83-96%
Heparin plasma(n=5) 85-100% 84-98% 95-101% 88-102%