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RVT(Resveratrol) ELISA Kit
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ELK8265
Size： Price：
48 T $265.00
96 T $376.00
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Overview

Product name: RVT(Resveratrol) ELISA Kit
Reactivity: General
Alternative Names: trans-3,5,4'-Trihydroxystilbene; 3,4',5-Stilbenetriol; trans-Resveratrol
Assay Type: Competitive Inhibition
Sensitivity: 91.1 ng/mL
Standard: 20000 ng/mL
Detection Range: 312.5-20000 ng/mL
Sample type: plant tissue and biological fluids
Assay length: 2h
Research Area: Cardiocerebral vascular system
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with RVT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to RVT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of RVT in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
20000.00 0.197
10000.00 0.336
5000.00 0.596
2500.00 0.913
1250.00 1.119
625.00 1.583
312.50 1.755
0.00 2.101

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant RVT and the recovery rates were calculated by comparing the measured value to the expected amount of RVT in samples.
Matrix Recovery range Average
serum(n=5) 79-93% 86%
EDTA plasma(n=5) 80-97% 88%
Heparin plasma(n=5) 96-109% 88%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of RVT and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 88-104% 86-102% 91-103% 87-90%
EDTA plasma(n=5) 81-94% 85-97% 93-102% 81-95%
Heparin plasma(n=5) 84-102% 85-93% 89-97% 95-102%