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Human Anti-GRIN2A(Anti-Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 2A Antibody) ELISA Kit
ELK8248
Size： Price：
48 T $320.00
96 T $458.00
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Overview

Product name: Human Anti-GRIN2A(Anti-Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 2A Antibody) ELISA Kit
Reactivity: Human
Alternative Names: NMDAR2A; NR2A; Glutamate [NMDA] receptor subunit epsilon-1; N-methyl D-aspartate receptor subtype 2A
Assay Type: Competitive Inhibition
Sensitivity: 1.3 ng/mL
Standard: 200 ng/mL
Detection Range: 3.13-200 ng/mL
Sample Type: serum, plasma and other biological fluids
Assay Length: 2.5h
Research Area: Neuro science;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human Anti-GRIN2A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Anti-GRIN2A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Anti-GRIN2A in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
200.00 0.155
100.00 0.386
50.00 0.524
25.00 0.812
12.50 1.139
6.25 1.557
3.13 1.697
0.00 2.112

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant Anti-GRIN2A and the recovery rates were calculated by comparing the measured value to the expected amount of Anti-GRIN2A in samples.
Matrix Recovery range Average
serum(n=5) 82-95% 88%
EDTA plasma(n=5) 88-102% 95%
Heparin plasma(n=5) 95-107% 101%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Anti-GRIN2A and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 98-106% 86-102% 88-96% 89-97%
EDTA plasma(n=5) 85-94% 87-103% 82-94% 78-90%
Heparin plasma(n=5) 85-94% 97-103% 79-91% 82-91%