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Horse COL1(Collagen Type I) ELISA Kit
ELK8222
Size： Price：
48 T $412.00
96 T $588.00
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Overview

Product name: Horse COL1(Collagen Type I) ELISA Kit
Reactivity: Horse
Alternative Names: COL1; COLI
Assay Type: Competitive Inhibition
Sensitivity: 7.29 ng/mL
Standard: 1500 ng/mL
Detection Range: 23.44-1500 ng/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 2h
Research Area: Metabolic pathway;Developmental science;Bone metabolism;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Horse COL1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Horse COL1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Horse COL1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
1500.00 0.189
750.00 0.411
375.00 0.651
187.50 0.913
93.75 1.157
46.88 1.592
23.44 1.813
0.00 2.226

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant COL1 and the recovery rates were calculated by comparing the measured value to the expected amount of COL1 in samples.
Matrix Recovery range Average
serum(n=5) 83-95% 89%
EDTA plasma(n=5) 90-105% 97%
Heparin plasma(n=5) 80-95% 88%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of COL1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 88-97% 86-92% 94-103% 89-97%
EDTA plasma(n=5) 85-94% 97-106% 82-95% 93-105%
Heparin plasma(n=5) 88-104% 96-105% 92-103% 82-96%