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Pig Anti-SST(Anti-Somatostatin Antibody) ELISA Kit
ELK8180
Size： Price：
48 T $412.00
96 T $588.00
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Overview

Product name: Pig Anti-SST(Anti-Somatostatin Antibody) ELISA Kit
Reactivity: Pig
Alternative Names: GHIH; SRIF; SMST; GHRIH; GHRIF; Growth Hormone Releasing Inhibiting Hormone; Somatotropin Release-Inhibiting Factor; Somatostatin-14; Somatostatin-28
Assay Type: Competitive Inhibition
Sensitivity: 1.26 ng/mL
Standard: 200 ng/mL
Detection Range: 3.13-200 ng/mL
Sample Type: serum, plasma and other biological fluids
Assay Length: 2.5h
Research Area: Tumor immunity;Endocrinology;Hormone metabolism;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Pig Anti-SST. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig Anti-SST. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig Anti-SST in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
200.00 0.191
100.00 0.386
50.00 0.655
25.00 0.878
12.50 1.139
6.25 1.578
3.13 1.796
0.00 2.232

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant Anti-SST and the recovery rates were calculated by comparing the measured value to the expected amount of Anti-SST in samples.
Matrix Recovery range Average
serum(n=5) 95-107% 101%
EDTA plasma(n=5) 90-105% 97%
Heparin plasma(n=5) 97-105% 101%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Anti-SST and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 88-97% 78-96% 80-99% 89-97%
EDTA plasma(n=5) 93-104% 87-96% 82-95% 78-99%
Heparin plasma(n=5) 95-104% 93-105% 82-101% 91-103%