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Human Anti-MOG(Anti-Myelin Oligodendrocyte Glycoprotein Antibody) ELISA Kit
ELK8083
Size： Price：
48 T $320.00
96 T $458.00
Manual MSDS Send email to us

Overview

Product name: Human Anti-MOG(Anti-Myelin Oligodendrocyte Glycoprotein Antibody) ELISA Kit
Reactivity: Human
Alternative Names: MOG Ab
Assay Type: Competitive Inhibition
Sensitivity: 1.15 ng/mL
Standard: 200 ng/mL
Detection Range: 3.13-200 ng/mL
Sample Type: serum, plasma and other biological fluids
Assay Length: 2.5h
Research Area: Infection immunity;Neuro science;Genetic science;Autoimmunity;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human Anti-MOG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Anti-MOG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Anti-MOG in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
200.00 0.197
100.00 0.310
50.00 0.527
25.00 0.846
12.50 1.389
6.25 1.457
3.13 1.761
0.00 2.475

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant Anti-MOG and the recovery rates were calculated by comparing the measured value to the expected amount of Anti-MOG in samples.
Matrix Recovery range Average
serum(n=5) 80-96% 88%
EDTA plasma(n=5) 78-95% 86%
Heparin plasma(n=5) 80-92% 86%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Anti-MOG and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 86-96% 91-107% 87-101% 95-102%
EDTA plasma(n=5) 92-103% 83-98% 88-97% 96-104%
Heparin plasma(n=5) 95-104% 93-102% 79-94% 87-98%