Products

PAF(Platelet Activating Factor) ELISA Kit
One-click to copy product information
ELK8072
Size： Price：
48 T $265.00
96 T $376.00
Manual MSDS Send email to us

Overview

Product name: PAF(Platelet Activating Factor) ELISA Kit
Reactivity: General
Alternative Names: PAF-Acether; AGEPC; Acetyl-Glyceryl-Ether-Phosphorylcholine
Assay Type: Competitive Inhibition
Sensitivity: 15 pg/mL
Standard: 3000 pg/mL
Detection Range: 46.88-3000 pg/mL
Sample type: serum, plasma and other biological fluids
Assay length: 2h
Research Area: Metabolic pathway;Infection immunity;Cardiovascular biology;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with PAF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to PAF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of PAF in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
3000.00 0.175
1500.00 0.389
750.00 0.672
375.00 0.856
187.50 1.225
93.75 1.587
46.88 1.762
0.00 2.101

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant PAF and the recovery rates were calculated by comparing the measured value to the expected amount of PAF in samples.
Matrix Recovery range Average
serum(n=5) 95-107% 101%
EDTA plasma(n=5) 78-90% 84%
Heparin plasma(n=5) 90-105% 92%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PAF and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 85-94% 90-99% 82-95% 92-105%
EDTA plasma(n=5) 85-97% 90-99% 82-101% 93-105%
Heparin plasma(n=5) 85-97% 86-102% 87-103% 85-101%