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Human Anti-AsAb(Anti-Sperm Antibody) ELISA Kit
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ELK8071
Size： Price：
48 T $320.00
96 T $458.00
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Overview

Product name: Human Anti-AsAb(Anti-Sperm Antibody) ELISA Kit
Reactivity: Human
Alternative Names: Anti-Spermatozoa Antibodies; Sperm Antibodies; Antisperm Antibodies
Assay Type: Sandwich
Sensitivity: 1.19 ng/mL
Standard: 200 ng/mL
Detection Range: 3.13-200 ng/mL
Sample type: serum, plasma and other biological fluids
Assay length: 3.5h
Research Area: Reproductive science;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Anti-AsAb. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Anti-AsAb. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Anti-AsAb, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Anti-AsAb in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
200.00 2.096 2.019
100.00 1.674 1.597
50.00 1.235 1.158
25.00 0.847 0.770
12.50 0.492 0.415
6.25 0.322 0.245
3.13 0.211 0.134
0.00 0.077 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant Anti-AsAb and the recovery rates were calculated by comparing the measured value to the expected amount of Anti-AsAb in samples.
Matrix Recovery range Average
serum(n=5) 78-92% 85%
EDTA plasma(n=5) 87-99% 93%
Heparin plasma(n=5) 85-99% 92%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Anti-AsAb and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 85-94% 90-99% 95-102% 82-90%
EDTA plasma(n=5) 90-99% 95-106% 87-101% 82-97%
Heparin plasma(n=5) 90-99% 95-106% 87-101% 82-97%