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Mouse KIBRA(Kidney And Brain Protein) ELISA Kit
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ELK8022
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48 T $320.00
96 T $458.00
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Overview

Product name: Mouse KIBRA(Kidney And Brain Protein) ELISA Kit
Reactivity: Mouse
Alternative Names: WWC1; WW Domain Containing Protein 1; WW,C2 And Coiled-Coil Domain Containing 1; HBeAg-binding protein 3
Assay Type: Sandwich
Sensitivity: 30 pg/mL
Standard: 5000 pg/mL
Detection Range: 78.13-5000 pg/mL
Sample type: Tissue homogenates, cell lysates and other biological fluids.
Assay length: 3.5h
Research Area: Signal transduction;
Uniprot ID: Q5SXA9
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse KIBRA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse KIBRA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse KIBRA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse KIBRA in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
5000.00 2.154 2.069
2500.00 1.563 1.478
1250.00 0.935 0.850
625.00 0.752 0.667
312.50 0.453 0.368
156.25 0.305 0.220
78.13 0.189 0.104
0.00 0.085 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant KIBRA and the recovery rates were calculated by comparing the measured value to the expected amount of KIBRA in samples.
Matrix Recovery range Average
serum(n=5) 80-92% 86%
EDTA plasma(n=5) 78-95% 86%
Heparin plasma(n=5) 80-96% 88%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of KIBRA and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 86-94% 93-102% 89-97% 85-94%
EDTA plasma(n=5) 95-102% 87-96% 78-92% 85-97%
Heparin plasma(n=5) 88-105% 86-92% 81-93% 79-97%