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Simian IL1R2(Interleukin 1 Receptor Type II) ELISA Kit
ELK7907
Size： Price：
48 T $412.00
96 T $588.00
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Overview

Product name: Simian IL1R2(Interleukin 1 Receptor Type II) ELISA Kit
Reactivity: Simian
Alternative Names: IL-1R2；CD121b; CD121-B; IL1-R2; IL1RB; Interleukin-1 Receptor Beta; CD121 Antigen-like Family Member B
Assay Type: Sandwich
Sensitivity: 0.205 ng/mL
Standard: 40 ng/mL
Detection Range: 0.63-40 ng/mL
Sample Type: Serum, plasma and other biological fluids.
Assay Length: 3.5h
Research Area: Signal transduction;CD & Adhesion molecule;Infection immunity;
Uniprot ID: Q29612
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Simian IL1R2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Simian IL1R2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Simian IL1R2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Simian IL1R2 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
40.00 2.204 2.119
20.00 1.628 1.543
10.00 1.144 1.059
5.00 0.882 0.797
2.50 0.533 0.448
1.25 0.385 0.300
0.63 0.168 0.083
0.00 0.085 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant IL1R2 and the recovery rates were calculated by comparing the measured value to the expected amount of IL1R2 in samples.
Matrix Recovery range Average
serum(n=5) 82-94% 88%
EDTA plasma(n=5) 84-98% 91%
Heparin plasma(n=5) 80-98% 89%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL1R2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 83-97% 92-103% 87-98% 93-101%
EDTA plasma(n=5) 92-105% 82-92% 86-94% 81-95%
Heparin plasma(n=5) 86-97% 88-95% 92-101% 87-98%