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rT3(Reverse Triiodothyronine) ELISA Kit
ELK7898
Size： Price：
48 T $265.00
96 T $376.00
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Overview

Product name: rT3(Reverse Triiodothyronine) ELISA Kit
Reactivity: General
Alternative Names: RT3; r-T3; Reverse T3; (2S)-2-Amino-3-[4-(4-Hydroxy-3,5-Diiodophenoxy)-3-Iodophenyl]propanoic Acid; 3,3',5'-Triiodo-L-thyronine
Assay Type: Competitive Inhibition
Sensitivity: 27.68 pg/mL
Standard: 6000 pg/mL
Detection Range: 93.75-6000 pg/mL
Sample Type: serum, plasma and other biological fluids
Assay Length: 2h
Research Area: Infection immunity;Endocrinology;Hormone metabolism;Autoimmunity;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with rT3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to rT3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of rT3 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
6000.00 0.183
3000.00 0.310
1500.00 0.527
750.00 0.598
375.00 0.763
187.50 1.457
93.75 1.724
0.00 2.236

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant rT3 and the recovery rates were calculated by comparing the measured value to the expected amount of rT3 in samples.
Matrix Recovery range Average
serum(n=5) 94-107% 100%
EDTA plasma(n=5) 87-95% 91%
Heparin plasma(n=5) 82-95% 101%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of rT3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 95-104% 82-93% 81-95% 91-102%
EDTA plasma(n=5) 89-97% 86-102% 94-106% 85-97%
Heparin plasma(n=5) 85-94% 83-92% 89-98% 85-94%