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Human Anti-IAA(Anti-Insulin Autoantibody Antibody) ELISA Kit
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ELK7844
Size： Price：
48 T $320.00
96 T $458.00
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Overview

Product name: Human Anti-IAA(Anti-Insulin Autoantibody Antibody) ELISA Kit
Reactivity: Human
Alternative Names: Anti-Insulin; Antiinsulin; Insulin Antibodies
Assay Type: Competitive Inhibition
Sensitivity: 1.12 ng/mL
Standard: 200 ng/mL
Detection Range: 3.13-200 ng/mL
Sample type: Serum, plasma and other biological fluids.
Assay length: 2.5h
Research Area: Signal transduction;Endocrinology;Autoimmunity;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human Anti-IAA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Anti-IAA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Anti-IAA in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
200.00 0.197
100.00 0.332
50.00 0.527
25.00 0.598
12.50 0.763
6.25 1.457
3.13 1.835
0.00 2.475

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant Anti-IAA and the recovery rates were calculated by comparing the measured value to the expected amount of Anti-IAA in samples.
Matrix Recovery range Average
serum(n=5) 80-92% 86%
EDTA plasma(n=5) 83-97% 90%
Heparin plasma(n=5) 86-99% 92%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Anti-IAA and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 82-96% 85-98% 95-104% 82-96%
EDTA plasma(n=5) 85-96% 87-101% 89-97% 85-94%
Heparin plasma(n=5) 98-105% 92-101% 85-105% 87-98%