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Rat CD30L(Cluster Of Differentiation 30 Ligand) Microsample ELISA Kit
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ELK7637MS
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48 T $320.00
96 T $458.00
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Overview

Product name: Rat CD30L(Cluster Of Differentiation 30 Ligand) Microsample ELISA Kit
Reactivity: Rat
Alternative Names: CD153; TNFSF8; CD30-L; Tumor Necrosis Factor Ligand Superfamily Member 8
Assay Type: Sandwich
Sensitivity: 49 pg/mL
Standard: 8000 pg/mL
Detection Range: 125-8000 pg/mL
Sample Type: serum, plasma, tissue homogenates and other biological fluids
Assay Length: 3.5h
Research Area: Signal transduction;CD & Adhesion molecule;Tumor immunity;Infection immunity;Immune molecule;
Uniprot ID: P32972
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat CD30L. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat CD30L. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat CD30L, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat CD30L in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
8000.00 2.107 2.014
4000.00 1.699 1.606
2000.00 1.085 0.992
1000.00 0.861 0.768
500.00 0.527 0.434
250.00 0.328 0.235
125.00 0.231 0.138
0.00 0.093 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant CD30L and the recovery rates were calculated by comparing the measured value to the expected amount of CD30L in samples.
Matrix Recovery range Average
serum(n=5) 95-108% 102%
EDTA plasma(n=5) 93-102% 98%
Heparin plasma(n=5) 87-113% 100%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CD30L and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 87-119% 81-109% 89-119% 93-115%
EDTA plasma(n=5) 94-104% 82-108% 84-112% 83-103%
Heparin plasma(n=5) 95-119% 81-93% 81-111% 92-118%

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