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Rat POMC(Proopiomelanocortin) ELISA Kit
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ELK7616
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48 T $320.00
96 T $458.00
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Overview

Product name: Rat POMC(Proopiomelanocortin) ELISA Kit
Reactivity: Rat
Alternative Names: POC; NPP; ProOMC; ACTH; Pro-OMC; Pro Opiomelanocortin; Corticotropin-lipotropin; Corticotropin; Adrenocorticotropic hormone
Assay Type: Competitive Inhibition
Sensitivity: 52.7 pg/mL
Standard: 10000 pg/mL
Detection Range: 156.25-10000 pg/mL
Sample type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length: 2h
Research Area: Endocrinology;
Uniprot ID: P01194
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat POMC. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat POMC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat POMC in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
10000.00 0.175
5000.00 0.367
2500.00 0.621
1250.00 0.861
625.00 1.197
312.50 1.578
156.25 1.799
0.00 2.132

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant POMC and the recovery rates were calculated by comparing the measured value to the expected amount of POMC in samples.
Matrix Recovery range Average
serum(n=5) 94-106% 100%
EDTA plasma(n=5) 80-97% 88%
Heparin plasma(n=5) 77-95% 91%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of POMC and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 86-94% 85-95% 87-99% 99-105%
EDTA plasma(n=5) 89-98% 83-103% 98-105% 92-101%
Heparin plasma(n=5) 87-96% 86-98% 92-103% 87-93%