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Human Ang1-9(Angiotensin 1-9) ELISA Kit
ELK7609
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48 T $265.00
96 T $376.00
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Overview

Product name: Human Ang1-9(Angiotensin 1-9) ELISA Kit
Reactivity: Human
Alternative Names: Ang1-9
Assay Type: Sandwich
Sensitivity: 5.31 pg/mL
Standard: 1000 pg/mL
Detection Range: 15.63-1000 pg/mL
Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area: Endocrinology;Cardiovascular biology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Ang1-9. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Ang1-9. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Ang1-9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Ang1-9 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
1000.00 2.274 2.188
500.00 1.621 1.535
250.00 1.217 1.131
125.00 0.937 0.851
62.50 0.611 0.525
31.25 0.336 0.250
15.63 0.236 0.150
0.00 0.086 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant Ang1-9 and the recovery rates were calculated by comparing the measured value to the expected amount of Ang1-9 in samples.
Matrix Recovery range Average
serum(n=5) 81-95% 88%
EDTA plasma(n=5) 95-103% 99%
Heparin plasma(n=5) 92-102% 97%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Ang1-9 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 80-97% 81-92% 87-103% 82-93%
EDTA plasma(n=5) 92-105% 85-101% 85-94% 87-96%
Heparin plasma(n=5) 91-101% 87-98% 89-103% 81-96%