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Mouse S17aH(17-Alpha-Hydroxylase) ELISA Kit
ELK7512
Size： Price：
48 T $320.00
96 T $458.00
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Overview

Product name: Mouse S17aH(17-Alpha-Hydroxylase) ELISA Kit
Reactivity: Mouse
Alternative Names: CYP17A1; CPT7; P450C17; 17-alpha-hydroxyprogesterone aldolase/17,20-Lyase; Steroid 17-alpha-monooxygenase; Cytochrome P450,Family 17,Subfamily A,Polypeptide 1
Assay Type: Sandwich
Sensitivity: 0.55 ng/mL
Standard: 100 ng/mL
Detection Range: 1.57-100 ng/mL
Sample Type: Tissue homogenates, cell lysates and other biological fluids
Assay Length: 3.5h
Research Area: Enzyme & Kinase;Metabolic pathway;Endocrinology;Cardiovascular biology;Hepatology;Gastroenterology;Nutrition metabolism;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse S17aH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse S17aH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse S17aH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse S17aH in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
100.00 2.204 2.118
50.00 1.769 1.683
25.00 1.073 0.987
12.50 0.757 0.671
6.25 0.559 0.473
3.13 0.365 0.279
1.57 0.175 0.089
0.00 0.086 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant S17aH and the recovery rates were calculated by comparing the measured value to the expected amount of S17aH in samples.
Matrix Recovery range Average
serum(n=5) 95-107% 101%
EDTA plasma(n=5) 87-99% 93%
Heparin plasma(n=5) 82-95% 88%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of S17aH and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 95-104% 82-96% 92-103% 90-99%
EDTA plasma(n=5) 89-98% 83-103% 98-105% 92-101%
Heparin plasma(n=5) 85-105% 87-98% 91-102% 86-102%