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Mouse REG1b(Regenerating Islet Derived Protein 1 Beta) ELISA Kit
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ELK6919
Size： Price：
48 T $320.00
96 T $458.00
96 T*5 $1947.00
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Overview

Product name: Mouse REG1b(Regenerating Islet Derived Protein 1 Beta) ELISA Kit
Reactivity: Mouse
Alternative Names: PSPS2; REGH; REGI-BETA; REGL; Pancreatic Stone Protein; Pancreatic Thread Protein; Lithostathine 1 Beta; Secretory Pancreatic Stone Protein 2
Assay Type: Sandwich
Sensitivity: 22.1 pg/mL
Standard: 4000 pg/mL
Detection Range: 62.5-4000 pg/mL
Sample Type: Serum, plasma and other biological fluids.
Assay Length: 3.5h
Research Area: Infection immunity;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse REG1b. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse REG1b. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse REG1b, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse REG1b in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
4000.00 2.368 2.295
2000.00 1.633 1.560
1000.00 1.067 0.994
500.00 0.752 0.679
250.00 0.538 0.465
125.00 0.318 0.245
62.50 0.186 0.113
0.00 0.073 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant REG1b and the recovery rates were calculated by comparing the measured value to the expected amount of REG1b in samples.
Matrix Recovery range Average
serum(n=5) 82-95% 88%
EDTA plasma(n=5) 78-91% 84%
Heparin plasma(n=5) 88-101% 94%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of REG1b and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 98-105% 92-101% 85-105% 87-98%
EDTA plasma(n=5) 88-102% 93-105% 91-99% 83-96%
Heparin plasma(n=5) 85-94% 87-96% 92-101% 82-95%