Products

Mouse PDGFC(Platelet Derived Growth Factor C) ELISA Kit
One-click to copy product information
ELK6247
Size： Price：
48 T $320.00
96 T $458.00
96 T*5 $1947.00
Manual MSDS Send email to us

Overview

Product name: Mouse PDGFC(Platelet Derived Growth Factor C) ELISA Kit
Reactivity: Mouse
Alternative Names: PDGF-C; SCDGF; VEGF-E; Fallotein; Spinal cord-derived growth factor; Platelet-derived growth factor C, latent form; Platelet-derived growth factor C, receptor-binding form
Assay Type: Sandwich
Sensitivity: 12.3 pg/mL
Standard: 2000 pg/mL
Detection Range: 31.25-2000 pg/mL
Sample Type: Plasma.
Assay Length: 3.5h
Research Area: Cytokine;
Uniprot ID: Q8CI19
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse PDGFC. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PDGFC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse PDGFC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse PDGFC in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
2000.00 1.952 1.866
1000.00 1.612 1.526
500.00 1.142 1.056
250.00 0.835 0.749
125.00 0.563 0.477
62.50 0.315 0.229
31.25 0.206 0.120
0.00 0.086 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant PDGFC and the recovery rates were calculated by comparing the measured value to the expected amount of PDGFC in samples.
Matrix Recovery range Average
serum(n=5) 77-95% 86%
EDTA plasma(n=5) 87-99% 93%
Heparin plasma(n=5) 78-92% 85%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PDGFC and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 83-97% 82-96% 86-98% 93-101%
EDTA plasma(n=5) 87-103% 79-92% 86-99% 84-97%
Heparin plasma(n=5) 88-97% 95-106% 83-98% 93-105%