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Pig NT-ProBNP(N-Terminal Pro-Brain Natriuretic Peptide) ELISA Kit
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ELK6163
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48 T $412.00
96 T $588.00
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Overview

Product name: Pig NT-ProBNP(N-Terminal Pro-Brain Natriuretic Peptide) ELISA Kit
Reactivity: Pig
Alternative Names: NT-Pro-BNP; ; N-BNP; ANFB_HUMAN; BNP(1-32); BNP(5-29); BNP-32; Gamma-brain natriuretic peptide; nppb
Assay Type: Sandwich
Sensitivity: 0.065 ng/mL
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample type: Serum, plasma and other biological fluids
Assay length: 3.5h
Research Area: Endocrinology;Cardiovascular biology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig NT-ProBNP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig NT-ProBNP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig NT-ProBNP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig NT-ProBNP in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
10.00 2.207 2.118
5.00 1.717 1.628
2.50 1.186 1.097
1.25 0.897 0.808
0.63 0.468 0.379
0.32 0.372 0.283
0.16 0.179 0.090
0.00 0.089 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant NT-ProBNP and the recovery rates were calculated by comparing the measured value to the expected amount of NT-ProBNP in samples.
Matrix Recovery range Average
serum(n=5) 80-95% 87%
EDTA plasma(n=5) 83-98% 90%
Heparin plasma(n=5) 86-99% 92%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NT-ProBNP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 81-94% 96-105% 92-103% 82-96%
EDTA plasma(n=5) 85-94% 87-101% 89-99% 85-95%
Heparin plasma(n=5) 83-98% 88-97% 96-105% 86-92%