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Pig F1+2(Prothrombin Fragment 1+2) ELISA Kit
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ELK6049
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48 T $412.00
96 T $588.00
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Overview

Product name: Pig F1+2(Prothrombin Fragment 1+2) ELISA Kit
Reactivity: Pig
Alternative Names: F1+2
Assay Type: Sandwich
Sensitivity: 2.6 ng/mL
Standard: 400 ng/mL
Detection Range: 6.25-400 ng/mL
Sample type: Serum, plasma and other biological fluids
Assay length: 3.5h
Research Area: Hematology
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig F1+2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig F1+2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig F1+2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig F1+2 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
400.00 2.266 2.179
200.00 1.536 1.449
100.00 1.035 0.948
50.00 0.847 0.760
25.00 0.468 0.381
12.50 0.387 0.300
6.25 0.252 0.165
0.00 0.087 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant F1+2 and the recovery rates were calculated by comparing the measured value to the expected amount of F1+2 in samples.
Matrix Recovery range Average
serum(n=5) 85-97% 91%
EDTA plasma(n=5) 79-93% 86%
Heparin plasma(n=5) 82-95% 88%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of F1+2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 96-105% 87-96% 90-98% 92-101%
EDTA plasma(n=5) 84-99% 81-95% 98-107% 96-101%
Heparin plasma(n=5) 88-95% 98-107% 90-99% 88-97%