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Rat TNFSF12(Tumor Necrosis Factor Ligand Superfamily, Member 12) ELISA Kit
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ELK6029
Size： Price：
48 T $320.00
96 T $458.00
96 T*5 $1947.00
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Overview

Product name: Rat TNFSF12(Tumor Necrosis Factor Ligand Superfamily, Member 12) ELISA Kit
Reactivity: Rat
Alternative Names: APO3L; DR3LG; TWEAK; APO3 ligand; TNF-related weak inducer of apoptosis
Assay Type: Sandwich
Sensitivity: 13.6 pg/mL
Standard: 2000 pg/mL
Detection Range: 31.25-2000 pg/mL
Sample Type: Serum, plasma, tissue homogenates, cell lysates and other biological fluids.
Assay Length: 3.5h
Research Area: Tumor immunity;
Uniprot ID: Q6AYC1
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat TNFSF12. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat TNFSF12. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat TNFSF12, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat TNFSF12 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
2000.00 2.132 2.047
1000.00 1.621 1.536
500.00 1.255 1.170
250.00 0.971 0.886
125.00 0.617 0.532
62.50 0.324 0.239
31.25 0.256 0.171
0.00 0.085 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant TNFSF12 and the recovery rates were calculated by comparing the measured value to the expected amount of TNFSF12 in samples.
Matrix Recovery range Average
serum(n=5) 92-106% 99%
EDTA plasma(n=5) 77-95% 86%
Heparin plasma(n=5) 88-103% 95%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TNFSF12 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 85-104% 87-106% 83-92% 82-90%
EDTA plasma(n=5) 85-96% 96-105% 81-93% 93-102%
Heparin plasma(n=5) 89-101% 84-101% 95-104% 88-105%