Products

Horse IL12A(Interleukin 12A) ELISA Kit
One-click to copy product information
ELK5876
Size： Price：
48 T $412.00
96 T $588.00
96 T*5 $2499.00
Manual MSDS Send email to us

Overview

Product name: Horse IL12A(Interleukin 12A) ELISA Kit
Reactivity: Horse
Alternative Names: IL-12A;p35; IL12-A; CLMF1; NFSK; NKSF1; Natural Killer Cell Stimulatory Factor 1; Cytotoxic Lymphocyte Maturation Factor 1; NF Cell Stimulatory Factor Chain 1
Assay Type: Sandwich
Sensitivity: 13.9 pg/mL
Standard: 2000 pg/mL
Detection Range: 31.25-2000 pg/mL
Sample Type: Serum, plasma and other biological fluids.
Assay Length: 3.5h
Research Area: Cytokine;Infection immunity;
Uniprot ID: Q9XSQ6
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Horse IL12A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Horse IL12A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Horse IL12A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Horse IL12A in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
2000.00 1.908 1.815
1000.00 1.497 1.404
500.00 1.073 0.980
250.00 0.726 0.633
125.00 0.457 0.364
62.50 0.312 0.219
31.25 0.206 0.113
0.00 0.093 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant IL12A and the recovery rates were calculated by comparing the measured value to the expected amount of IL12A in samples.
Matrix Recovery range Average
serum(n=5) 81-95% 88%
EDTA plasma(n=5) 83-97% 90%
Heparin plasma(n=5) 87-95% 91%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL12A and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 92-105% 83-92% 89-98% 85-94%
EDTA plasma(n=5) 87-96% 86-98% 91-103% 87-90%
Heparin plasma(n=5) 81-94% 85-97% 93-102% 81-95%