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Rat IL-2sRa/CD25(Soluble Interleukin-2 Receptor alpha chain) Microsample ELISA Kit
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ELK5694MS
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48 T $320.00
96 T $458.00
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Overview

Product name: Rat IL-2sRa/CD25(Soluble Interleukin-2 Receptor alpha chain) Microsample ELISA Kit
Reactivity: Rat
Alternative Names: IL-2RA;CD25; IL2R-a; IL2-RA; IDDM10; IL2R; TCGFR; p55; TAC antigen; IL2Ra; Interleukin 2 Receptor Alpha
Assay Type: Sandwich
Sensitivity: 0.06 ng/mL
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates and other biological fluids
Assay Length: 3.5h
Research Area: Signal transduction;CD & Adhesion molecule;Tumor immunity;Infection immunity;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat IL-2sRa/CD25. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat IL-2sRa/CD25. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat IL-2sRa/CD25, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat IL-2sRa/CD25 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
10.00 2.399 2.304
5.00 1.665 1.570
2.50 1.197 1.102
1.25 0.937 0.842
0.63 0.527 0.432
0.31 0.368 0.273
0.16 0.200 0.105
0.00 0.095 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant IL-2sRa/CD25 and the recovery rates were calculated by comparing the measured value to the expected amount of IL-2sRa/CD25 in samples.
Matrix Recovery range Average
serum(n=5) 91-118% 105%
EDTA plasma(n=5) 87-107% 97%
Heparin plasma(n=5) 83-88% 86%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-2sRa/CD25 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 93-115% 94-114% 87-106% 81-112%
EDTA plasma(n=5) 87-96% 88-107% 88-113% 90-96%
Heparin plasma(n=5) 87-105% 83-108% 95-110% 89-94%

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