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Rat sIL-6R(SolubleInterleukin 6 Receptor) Microsample ELISA Kit
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ELK5684MS
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48 T $320.00
96 T $458.00
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Overview

Product name: Rat sIL-6R(SolubleInterleukin 6 Receptor) Microsample ELISA Kit
Reactivity: Rat
Alternative Names: IL-6R;CD126; gp80; IL6R; IL-6R-1; IL-6R-Alpha; IL6RA; IL-6 receptor subunit alpha; Membrane glycoprotein 80; IL6R; Interleukin 6 Receptor
Assay Type: Sandwich
Sensitivity: 0.06 ng/mL
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: serum, plasma, tissue homogenates and other biological fluids
Assay Length: 3.5h
Research Area: Signal transduction;CD & Adhesion molecule;Infection immunity;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat sIL-6R. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat sIL-6R. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat sIL-6R, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat sIL-6R in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
10.00 2.225 2.121
5.00 1.773 1.669
2.50 1.128 1.024
1.25 0.902 0.798
0.63 0.566 0.462
0.31 0.382 0.278
0.16 0.177 0.073
0.00 0.104 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant sIL-6R and the recovery rates were calculated by comparing the measured value to the expected amount of sIL-6R in samples.
Matrix Recovery range Average
serum(n=5) 82-108% 95%
EDTA plasma(n=5) 93-112% 103%
Heparin plasma(n=5) 84-108% 96%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of sIL-6R and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 92-118% 91-107% 90-97% 89-109%
EDTA plasma(n=5) 84-91% 90-114% 85-109% 81-103%
Heparin plasma(n=5) 91-118% 93-104% 85-104% 87-117%

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