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Mouse ProNT/NMN(Proneurotensin/neuromedin N) ELISA Kit
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ELK5591
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48 T $320.00
96 T $458.00
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Overview

Product name: Mouse ProNT/NMN(Proneurotensin/neuromedin N) ELISA Kit
Reactivity: Mouse
Alternative Names: Proneurotensin 1-117
Assay Type: Competitive Inhibition
Sensitivity: 5.4 pg/mL
Standard: 1000 pg/mL
Detection Range: 15.63-1000 pg/mL
Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length: 2h
Research Area: Neuro science;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse ProNT/NMN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse ProNT/NMN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse ProNT/NMN in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
1000.00 0.185
500.00 0.389
250.00 0.557
125.00 0.861
62.50 1.119
31.25 1.565
15.63 1.755
0.00 2.132

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant ProNT/NMN and the recovery rates were calculated by comparing the measured value to the expected amount of ProNT/NMN in samples.
Matrix Recovery range Average
serum(n=5) 80-95% 87%
EDTA plasma(n=5) 95-107% 101%
Heparin plasma(n=5) 80-93% 88%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ProNT/NMN and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 95-104% 85-96% 95-102% 87-98%
EDTA plasma(n=5) 79-93% 86-92% 84-98% 89-102%
Heparin plasma(n=5) 96-101% 85-92% 79-96% 89-100%