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Human IL2Rb(Interleukin 2 Receptor Beta) Microsample ELISA Kit
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ELK5053MS
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48 T $320.00
96 T $458.00
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Overview

Product name: Human IL2Rb(Interleukin 2 Receptor Beta) Microsample ELISA Kit
Reactivity: Human
Alternative Names: CD122; IL2R-b; IL2-RB; P70-75; High affinity IL-2 receptor subunit beta; Interleukin-2 receptor subunit beta; IL-2 receptor subunit beta
Assay Type: Sandwich
Sensitivity: 30 pg/mL
Standard: 5000 pg/mL
Detection Range: 78.13-5000 pg/mL
Sample Type: Serum, plasma, tissue homogenates and other biological fluids.
Assay Length: 3.5h
Research Area: CD & Adhesion molecule;Infection immunity;
Uniprot ID: P14784
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IL2Rb. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IL2Rb. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IL2Rb, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IL2Rb in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
5000.00 2.168 2.079
2500.00 1.572 1.483
1250.00 1.210 1.121
625.00 0.952 0.863
312.50 0.495 0.406
156.25 0.321 0.232
78.13 0.197 0.108
0.00 0.089 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant IL2Rb and the recovery rates were calculated by comparing the measured value to the expected amount of IL2Rb in samples.
Matrix Recovery range Average
serum(n=5) 83-106% 95%
EDTA plasma(n=5) 83-99% 91%
Heparin plasma(n=5) 82-115% 99%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL2Rb and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 93-103% 91-98% 91-113% 85-100%
EDTA plasma(n=5) 85-97% 93-102% 80-106% 83-90%
Heparin plasma(n=5) 80-119% 85-119% 86-105% 90-106%

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