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Human M6PR(Mannose-6-Phosphate Receptor) ELISA Kit
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ELK5048
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48 T $320.00
96 T $458.00
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Overview

Product name: Human M6PR(Mannose-6-Phosphate Receptor) ELISA Kit
Reactivity: Human
Alternative Names: SMPR; MPR46; MPRD; Cation Dependent Mannose 6-Phosphate Receptor; 46 kDa mannose 6-phosphate receptor
Assay Type: Sandwich
Sensitivity: 0.63 ng/mL
Standard: 100 ng/mL
Detection Range: 1.57-100 ng/mL
Sample type: Serum, plasma, tissue homogenates and other biological fluids.
Assay length: 3.5h
Research Area: CD & Adhesion molecule;
Uniprot ID: P20645
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human M6PR. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human M6PR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human M6PR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human M6PR in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
100.00 2.401 2.312
50.00 1.537 1.448
25.00 1.197 1.108
12.50 0.752 0.663
6.25 0.567 0.478
3.13 0.354 0.265
1.57 0.124 0.035
0.00 0.089 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant M6PR and the recovery rates were calculated by comparing the measured value to the expected amount of M6PR in samples.
Matrix Recovery range Average
serum(n=5) 80-96% 88%
EDTA plasma(n=5) 78-95% 86%
Heparin plasma(n=5) 80-92% 86%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of M6PR and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 92-104% 82-96% 95-102% 87-98%
EDTA plasma(n=5) 85-97% 87-96% 82-95% 92-105%
Heparin plasma(n=5) 87-96% 92-105% 89-102% 92-101%