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Human CCK12(Cholecystokinin 12) ELISA Kit
ELK4876
Size： Price：
48 T $320.00
96 T $458.00
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Overview

Product name: Human CCK12(Cholecystokinin 12) ELISA Kit
Reactivity: Human
Alternative Names: CCK12
Assay Type: Competitive Inhibition
Sensitivity: 4.09 pg/mL
Standard: 1000 pg/mL
Detection Range: 15.63-1000 pg/mL
Sample Type: Serum, plasma and other biological fluids.
Assay Length: 2h
Research Area: Endocrinology;Gastroenterology;Hormone metabolism;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human CCK12. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CCK12. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CCK12 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
1000.00 0.217
500.00 0.467
250.00 0.722
125.00 0.997
62.50 1.236
31.25 1.596
15.63 1.889
0.00 2.197

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant CCK12 and the recovery rates were calculated by comparing the measured value to the expected amount of CCK12 in samples.
Matrix Recovery range Average
serum(n=5) 83-95% 89%
EDTA plasma(n=5) 82-94% 88%
Heparin plasma(n=5) 85-97% 85%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CCK12 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 86-94% 85-95% 87-99% 99-105%
EDTA plasma(n=5) 80-101% 87-96% 96-105% 95-103%
Heparin plasma(n=5) 85-103% 82-96% 87-98% 93-101%