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Human y+LAT1(L-Type Amino Acid Transporter 1) ELISA Kit
ELK4710
Size： Price：
48 T $320.00
96 T $458.00
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Overview

Product name: Human y+LAT1(L-Type Amino Acid Transporter 1) ELISA Kit
Reactivity: Human
Alternative Names: SLC7A7; LPI; Y+LAT1; MOP-2; Monocyte amino acid permease 2; Y+L Amino Acid Transporter 1; Solute Carrier Family 7 Member 7,Cationic Amino Acid Transporter,y+ System
Assay Type: Sandwich
Sensitivity: 0.118 ng/mL
Standard: 20 ng/mL
Detection Range: 0.32-20 ng/mL
Sample Type: Tissue homogenates, cell lysates and other biological fluids.
Assay Length: 3.5h
Research Area: Signal transduction;
Uniprot ID: Q9UM01
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human y+LAT1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human y+LAT1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human y+LAT1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human y+LAT1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
20.00 2.153 2.049
10.00 1.562 1.458
5.00 1.144 1.040
2.50 0.877 0.773
1.25 0.593 0.489
0.63 0.239 0.135
0.32 0.187 0.083
0.00 0.104 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant y+LAT1 and the recovery rates were calculated by comparing the measured value to the expected amount of y+LAT1 in samples.
Matrix Recovery range Average
serum(n=5) 89-103% 96%
EDTA plasma(n=5) 86-99% 92%
Heparin plasma(n=5) 83-97% 90%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of y+LAT1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 88-99% 81-93% 89-97% 85-94%
EDTA plasma(n=5) 87-96% 82-95% 93-102% 87-96%
Heparin plasma(n=5) 93-106% 87-99% 85-93% 79-96%