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Mouse CDHH(Cadherin, Heart) Microsample ELISA Kit
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ELK4662MS
Size： Price：
48 T $320.00
96 T $458.00
96 T*5 $1947.00
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Overview

Product name: Mouse CDHH(Cadherin, Heart) Microsample ELISA Kit
Reactivity: Mouse
Alternative Names: CDH13; P105; T-cad; Cadherin 13; T-Cadherin; H-Cadherin; Truncated cadherin
Assay Type: Sandwich
Sensitivity: 22.8 pg/mL
Standard: 4000 pg/mL
Detection Range: 62.5-4000 pg/mL
Sample Type: Serum, plasma and other biological fluids.
Assay Length: 3.5h
Research Area: CD & Adhesion molecule;Tumor immunity;Infection immunity;Cardiovascular biology;
Uniprot ID: Q9WTR5
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse CDHH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse CDHH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse CDHH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse CDHH in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
4000.00 1.950 1.870
2000.00 1.540 1.460
1000.00 1.177 1.097
500.00 0.764 0.684
250.00 0.560 0.480
125.00 0.344 0.264
62.50 0.200 0.120
0.00 0.080 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant CDHH and the recovery rates were calculated by comparing the measured value to the expected amount of CDHH in samples.
Matrix Recovery range Average
serum(n=5) 93-112% 103%
EDTA plasma(n=5) 86-116% 101%
Heparin plasma(n=5) 95-110% 103%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CDHH and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 86-100% 89-103% 86-93% 84-119%
EDTA plasma(n=5) 82-107% 91-110% 92-119% 80-108%
Heparin plasma(n=5) 92-113% 82-108% 94-108% 82-90%

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