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Human APLN(Apelin) ELISA Kit
ELK4069
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48 T $320.00
96 T $458.00
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OverviewPublications

Product name: Human APLN(Apelin) ELISA Kit
Reactivity: Human
Alternative Names: XNPEP2; APEL; APJ endogenous ligand
Assay Type: Competitive Inhibition
Sensitivity: 8.4 pg/mL
Standard: 1600 pg/mL
Detection Range: 25-1600 pg/mL
Sample Type: serum, plasma and other biological fluids
Assay Length: 2h
Research Area: Endocrinology;
Uniprot ID: Q9ULZ1
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human APLN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human APLN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human APLN in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
1600.00 0.195
800.00 0.397
400.00 0.451
200.00 0.899
100.00 1.236
50.00 1.589
25.00 1.863
0.00 2.232

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant APLN and the recovery rates were calculated by comparing the measured value to the expected amount of APLN in samples.
Matrix Recovery range Average
serum(n=5) 80-92% 86%
EDTA plasma(n=5) 80-96% 88%
Heparin plasma(n=5) 78-95% 87%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of APLN and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 85-105% 87-98% 85-96% 87-101%
EDTA plasma(n=5) 87-96% 85-98% 89-102% 85-98%
Heparin plasma(n=5) 85-92% 79-94% 83-96% 85-106%