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Rat F1+2(Prothrombin Fragment 1+2) ELISA Kit
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ELK3966
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48 T $320.00
96 T $458.00
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Overview

Product name: Rat F1+2(Prothrombin Fragment 1+2) ELISA Kit
Reactivity: Rat
Alternative Names: F1+2
Assay Type: Sandwich
Sensitivity: 1.36 ng/mL
Standard: 200 ng/mL
Detection Range: 3.13-200 ng/mL
Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length: 3.5h
Research Area: Hematology
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat F1+2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat F1+2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat F1+2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat F1+2 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
200.00 2.268 2.174
100.00 1.608 1.514
50.00 1.251 1.157
25.00 0.834 0.740
12.50 0.521 0.427
6.25 0.331 0.237
3.13 0.175 0.081
0.00 0.094 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant F1+2 and the recovery rates were calculated by comparing the measured value to the expected amount of F1+2 in samples.
Matrix Recovery range Average
serum(n=5) 87-95% 91%
EDTA plasma(n=5) 93-107% 100%
Heparin plasma(n=5) 87-99% 93%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of F1+2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 92-101% 87-98% 85-92% 79-96%
EDTA plasma(n=5) 78-92% 96-105% 87-98% 95-102%
Heparin plasma(n=5) 85-94% 87-101% 86-97% 88-95%