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Human CLEC2C(C-Type Lectin Domain Family 2, Member C) ELISA Kit
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ELK3755
Size： Price：
48 T $320.00
96 T $458.00
96 T*5 $1947.00
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Overview

Product name: Human CLEC2C(C-Type Lectin Domain Family 2, Member C) ELISA Kit
Reactivity: Human
Alternative Names: CD69; CLEC2-C; AIM; p60; EA1; MLR-3; Early T-Cell Activation Antigen p60; Early activation antigen CD69; Activation inducer molecule; Leukocyte surface antigen Leu-23
Assay Type: Sandwich
Sensitivity: 6 pg/mL
Standard: 1000 pg/mL
Detection Range: 15.63-1000 pg/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates and other biological fluids
Assay Length: 3.5h
Research Area: Signal transduction;CD & Adhesion molecule;Immune molecule;
Uniprot ID: Q07108
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CLEC2C. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CLEC2C. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CLEC2C, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CLEC2C in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
1000.00 2.285 2.190
500.00 1.771 1.676
250.00 1.157 1.062
125.00 0.857 0.762
62.50 0.593 0.498
31.25 0.386 0.291
15.63 0.225 0.130
0.00 0.095 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant CLEC2C and the recovery rates were calculated by comparing the measured value to the expected amount of CLEC2C in samples.
Matrix Recovery range Average
serum(n=5) 80-95% 87%
EDTA plasma(n=5) 84-102% 93%
Heparin plasma(n=5) 87-95% 90%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CLEC2C and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 89-102% 92-101% 88-102% 87-102%
EDTA plasma(n=5) 85-92% 79-96% 89-101% 85-97%
Heparin plasma(n=5) 86-101% 86-101% 87-96% 92-105%