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Human IFNa/bR2(Interferon Alpha/Beta Receptor 2) ELISA Kit
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ELK3549
Size： Price：
48 T $320.00
96 T $458.00
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Overview

Product name: Human IFNa/bR2(Interferon Alpha/Beta Receptor 2) ELISA Kit
Reactivity: Human
Alternative Names: IFNαR2; IFN-alpha-REC; IFN-R; IFNαBR; IFNαRB; Interferon(Alpha,Beta And Omega)Receptor Beta Chain; Interferon alpha binding protein; Type I interferon receptor 2
Assay Type: Sandwich
Sensitivity: 0.25 ng/mL
Standard: 40 ng/mL
Detection Range: 0.63-40 ng/mL
Sample type: Serum, plasma, urine and other biological fluids.
Assay length: 3.5h
Research Area: Infection immunity;
Uniprot ID: P48551
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IFNa/bR2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IFNa/bR2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IFNa/bR2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IFNa/bR2 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
40.00 2.045 1.941
20.00 1.527 1.423
10.00 1.175 1.071
5.00 0.897 0.793
2.50 0.563 0.459
1.25 0.426 0.322
0.63 0.213 0.109
0.00 0.104 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant IFNa/bR2 and the recovery rates were calculated by comparing the measured value to the expected amount of IFNa/bR2 in samples.
Matrix Recovery range Average
serum(n=5) 92-106% 99%
EDTA plasma(n=5) 87-95% 91%
Heparin plasma(n=5) 78-92% 85%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IFNa/bR2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 86-94% 93-102% 89-97% 85-94%
EDTA plasma(n=5) 79-96% 85-99% 79-93% 89-97%
Heparin plasma(n=5) 85-94% 87-101% 89-99% 85-95%