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Human CCR3(Chemokine C-C-Motif Receptor 3) Microsample ELISA Kit
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ELK2980MS
Size： Price：
48 T $320.00
96 T $458.00
96 T*5 $1947.00
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Overview

Product name: Human CCR3(Chemokine C-C-Motif Receptor 3) Microsample ELISA Kit
Reactivity: Human
Alternative Names: CD193; CC-CKR3; C-C CKR-3; CMKBR3; CKR3; Eosinophil eotaxin receptor
Assay Type: Sandwich
Sensitivity: 13.1 pg/mL
Standard: 2000 pg/mL
Detection Range: 31.25-2000 pg/mL
Sample Type: Tissue homogenates, cell lysates and other biological fluids.
Assay Length: 3.5h
Research Area: CD & Adhesion molecule;Tumor immunity;Infection immunity;
Uniprot ID: P51677
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CCR3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CCR3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CCR3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CCR3 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
2000.00 2.196 2.104
1000.00 1.460 1.368
500.00 1.217 1.125
250.00 0.892 0.800
125.00 0.473 0.381
62.50 0.385 0.293
31.25 0.227 0.135
0.00 0.092 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant CCR3 and the recovery rates were calculated by comparing the measured value to the expected amount of CCR3 in samples.
Matrix Recovery range Average
serum(n=5) 88-103% 96%
EDTA plasma(n=5) 81-125% 103%
Heparin plasma(n=5) 93-119% 106%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CCR3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 92-117% 88-100% 94-99% 82-109%
EDTA plasma(n=5) 80-90% 92-112% 93-111% 88-104%
Heparin plasma(n=5) 81-91% 85-101% 82-94% 95-114%

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