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Chicken MIP1b(Macrophage Inflammatory Protein 1 Beta) ELISA Kit
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ELK2878
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48 T $412.00
96 T $588.00
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Overview

Product name: Chicken MIP1b(Macrophage Inflammatory Protein 1 Beta) ELISA Kit
Reactivity: Chicken
Alternative Names: MIP1 Beta; CCL4; ACT2; G-26; LAG1; MIP1-B; SCYA4; Chemokine C-C-Motif Ligand 4; Small Inducible Cytokine A4; Homologous To Mouse Mip-1b; CCL4L1; C C motif chemokine ligand 4 like 1; CC chemokine ligand 4L1; C-C motif chemokine 4-like; Lymphocyte activation gene 1 protein; Macrophage inflammatory protein 1-beta; Monocyte adherence-induced protein 5-alpha; Small-inducible cytokine A4-like; CCL4L; SCYA4L1; CCL4L2; SCYA4L2
Assay Type: Sandwich
Sensitivity: 14.3 pg/mL
Standard: 2000 pg/mL
Detection Range: 31.25-2000 pg/mL
Sample type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Assay length: 3.5h
Research Area: Cytokine;Infection immunity;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chicken MIP1b. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Chicken MIP1b. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chicken MIP1b, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chicken MIP1b in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
2000.00 2.153 2.045
1000.00 1.628 1.520
500.00 1.217 1.109
250.00 0.697 0.589
125.00 0.625 0.517
62.50 0.379 0.271
31.25 0.174 0.066
0.00 0.108 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant MIP1b and the recovery rates were calculated by comparing the measured value to the expected amount of MIP1b in samples.
Matrix Recovery range Average
serum(n=5) 78-90% 84%
EDTA plasma(n=5) 84-97% 90%
Heparin plasma(n=5) 87-95% 91%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of MIP1b and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 81-94% 85-97% 93-102% 81-95%
EDTA plasma(n=5) 85-92% 83-101% 87-98% 79-92%
Heparin plasma(n=5) 85-96% 86-98% 92-103% 87-93%