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Mouse PK2(Prokineticin 2) ELISA Kit
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ELK2817
Size： Price：
48 T $320.00
96 T $458.00
96 T*5 $1947.00
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Overview

Product name: Mouse PK2(Prokineticin 2) ELISA Kit
Reactivity: Mouse
Alternative Names: PK-2; Bv8; MIT1; KAL4; Protein Bv8 Homolog
Assay Type: Competitive Inhibition
Sensitivity: 94.4 pg/mL
Standard: 20000 pg/mL
Detection Range: 312.5-20000 pg/mL
Sample Type: Serum, plasma and other biological fluids
Assay Length: 2h
Research Area: Neuro science;
Uniprot ID: Q9QXU7
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse PK2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PK2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse PK2 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
20000.00 0.191
10000.00 0.396
5000.00 0.621
2500.00 0.861
1250.00 1.119
625.00 1.522
312.50 1.799
0.00 2.132

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant PK2 and the recovery rates were calculated by comparing the measured value to the expected amount of PK2 in samples.
Matrix Recovery range Average
serum(n=5) 83-99% 95%
EDTA plasma(n=5) 85-97% 91%
Heparin plasma(n=5) 97-105% 101%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PK2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 80-101% 87-96% 86-97% 88-95%
EDTA plasma(n=5) 85-92% 79-96% 92-101% 87-98%
Heparin plasma(n=5) 95-103% 89-96% 87-98% 85-93%