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Rat I-PTH(IntactParathormone) ELISA Kit
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ELK2563
Size： Price：
48 T $320.00
96 T $458.00
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Overview

Product name: Rat I-PTH(IntactParathormone) ELISA Kit
Reactivity: Rat
Alternative Names: iPTH; Intact Parathyroid Hormone; Parathormone; Parathyrin; PTH; Parathyroid Hormone
Assay Type: Sandwich
Sensitivity: 3.76 pg/mL
Standard: 800 pg/mL
Detection Range: 12.5-800 pg/mL
Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length: 3.5h
Research Area: Metabolic pathway;Endocrinology;Hormone metabolism;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat I-PTH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat I-PTH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat I-PTH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat I-PTH in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
800.00 2.532 2.446
400.00 1.593 1.507
200.00 1.121 1.035
100.00 0.852 0.766
50.00 0.548 0.462
25.00 0.385 0.299
12.50 0.258 0.172
0.00 0.086 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant I-PTH and the recovery rates were calculated by comparing the measured value to the expected amount of I-PTH in samples.
Matrix Recovery range Average
serum(n=5) 80-95% 87%
EDTA plasma(n=5) 78-96% 87%
Heparin plasma(n=5) 78-92% 85%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of I-PTH and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 85-94% 79-92% 82-97% 92-101%
EDTA plasma(n=5) 85-92% 80-96% 93-105% 90-99%
Heparin plasma(n=5) 78-92% 91-96% 85-93% 95-101%