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Rat ET-1(Endothelin1) Microsample ELISA Kit
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ELK2436MS
Size： Price：
48 T $320.00
96 T $458.00
96 T*5 $1947.00
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Overview

Product name: Rat ET-1(Endothelin1) Microsample ELISA Kit
Reactivity: Rat
Alternative Names: ET1; PPET1; Preproendothelin-1; Big endothelin-1; EDN1
Assay Type: Sandwich
Sensitivity: 2.67 pg/mL
Standard: 500 pg/mL
Detection Range: 7.82-500 pg/mL
Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area: Endocrinology;Cardiovascular biology;
Uniprot ID: P22387
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat ET-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat ET-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat ET-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat ET-1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
500.00 2.001 1.911
250.00 1.558 1.468
125.00 1.067 0.977
62.50 0.704 0.614
31.25 0.599 0.509
15.63 0.300 0.210
7.81 0.211 0.121
0.00 0.090 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant ET-1 and the recovery rates were calculated by comparing the measured value to the expected amount of ET-1 in samples.
Matrix Recovery range Average
serum(n=5) 86-101% 94%
EDTA plasma(n=5) 84-90% 87%
Heparin plasma(n=5) 82-115% 99%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ET-1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 95-109% 90-104% 83-105% 82-104%
EDTA plasma(n=5) 81-105% 88-95% 90-103% 86-114%
Heparin plasma(n=5) 82-109% 88-102% 90-95% 84-95%

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