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Human IL-2sRa/CD25(Soluble Interleukin-2 Receptor alpha chain) Microsample ELISA Kit
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ELK2178MS
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48 T $320.00
96 T $458.00
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Overview

Product name: Human IL-2sRa/CD25(Soluble Interleukin-2 Receptor alpha chain) Microsample ELISA Kit
Reactivity: Human
Alternative Names: IL-2RA;CD25; IL2R-a; IL2-RA; IDDM10; IL2R; TCGFR; p55; TAC antigen; IL2Ra; Interleukin 2 Receptor Alpha
Assay Type: Sandwich
Sensitivity: 6.5 pg/mL
Standard: 1000 pg/mL
Detection Range: 15.63-1000 pg/mL
Sample Type: Serum, plasma, tissue homogenates and other biological fluids
Assay Length: 3.5h
Research Area: Signal transduction;CD & Adhesion molecule;Tumor immunity;Infection immunity;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IL-2sRa/CD25. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IL-2sRa/CD25. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IL-2sRa/CD25, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IL-2sRa/CD25 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
1000.00 2.074 1.985
500.00 1.517 1.428
250.00 1.172 1.083
125.00 0.958 0.869
62.50 0.504 0.415
31.25 0.317 0.228
15.63 0.190 0.101
0.00 0.089 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant IL-2sRa/CD25 and the recovery rates were calculated by comparing the measured value to the expected amount of IL-2sRa/CD25 in samples.
Matrix Recovery range Average
serum(n=5) 83-93% 88%
EDTA plasma(n=5) 81-125% 103%
Heparin plasma(n=5) 86-95% 91%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-2sRa/CD25 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 82-109% 81-101% 94-113% 84-118%
EDTA plasma(n=5) 84-95% 89-97% 86-106% 84-94%
Heparin plasma(n=5) 89-119% 82-94% 95-116% 80-120%

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