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Human F1+2(Prothrombin Fragment 1+2) ELISA Kit
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ELK2028
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48 T $320.00
96 T $458.00
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Overview

Product name: Human F1+2(Prothrombin Fragment 1+2) ELISA Kit
Reactivity: Human
Alternative Names: F1+2
Assay Type: Sandwich
Sensitivity: 0.36 ng/mL
Standard: 50 ng/mL
Detection Range: 0.79-50 ng/mL
Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length: 3.5h
Research Area: Hematology
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human F1+2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human F1+2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human F1+2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human F1+2 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
50.00 1.964 1.869
25.00 1.616 1.521
12.50 1.052 0.957
6.25 0.733 0.638
3.13 0.548 0.453
1.57 0.315 0.220
0.79 0.230 0.135
0.00 0.095 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant F1+2 and the recovery rates were calculated by comparing the measured value to the expected amount of F1+2 in samples.
Matrix Recovery range Average
serum(n=5) 80-95% 87%
EDTA plasma(n=5) 85-97% 91%
Heparin plasma(n=5) 96-108% 102%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of F1+2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 95-104% 93-102% 79-94% 87-98%
EDTA plasma(n=5) 86-93% 91-103% 87-98% 95-101%
Heparin plasma(n=5) 83-96% 86-95% 90-102% 87-98%