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Human CYFRA21-1(Cytokeratin Fragment Antigen 21-1) Microsample ELISA Kit
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ELK1967MS
Size： Price：
48 T $320.00
96 T $458.00
96 T*5 $1947.00
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Overview

Product name: Human CYFRA21-1(Cytokeratin Fragment Antigen 21-1) Microsample ELISA Kit
Reactivity: Human
Alternative Names: CYFRA21-1
Assay Type: Sandwich
Sensitivity: 0.117 ng/mL
Standard: 20 ng/mL
Detection Range: 0.32-20 ng/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area: Tumor immunity;Dermatology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CYFRA21-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CYFRA21-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CYFRA21-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CYFRA21-1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
20.00 2.067 1.975
10.00 1.523 1.431
5.00 1.015 0.923
2.50 0.894 0.802
1.25 0.535 0.443
0.63 0.397 0.305
0.31 0.203 0.111
0.00 0.092 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant CYFRA21-1 and the recovery rates were calculated by comparing the measured value to the expected amount of CYFRA21-1 in samples.
Matrix Recovery range Average
serum(n=5) 84-98% 91%
EDTA plasma(n=5) 93-123% 108%
Heparin plasma(n=5) 82-110% 96%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CYFRA21-1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 85-117% 87-99% 90-98% 93-117%
EDTA plasma(n=5) 90-112% 90-96% 87-102% 91-103%
Heparin plasma(n=5) 83-94% 80-109% 93-113% 89-114%

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