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Human C4B(Complement C4-B) ELISA Kit
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ELK1872
Size： Price：
48 T $320.00
96 T $458.00
96 T*5 $1947.00
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Product name: Human C4B(Complement C4-B) ELISA Kit
Reactivity: Human
Alternative Names: CO4; CPAMD3; Basic complement C4; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 3
Assay Type: Sandwich
Sensitivity: 2.3 ng/mL
Standard: 300 ng/mL
Detection Range: 4.69-300 ng/mL
Sample Type: serum, plasma and other biological fluids
Assay Length: 3.5h
Research Area: Metabolic pathway;Infection immunity;Immune molecule;
Uniprot ID: P0C0L5
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human C4B. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human C4B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human C4B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human C4B in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
300.00 2.058 1.970
150.00 1.674 1.586
75.00 1.128 1.040
37.50 0.821 0.733
18.75 0.518 0.430
9.38 0.262 0.174
4.69 0.216 0.128
0.00 0.088 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant C4B and the recovery rates were calculated by comparing the measured value to the expected amount of C4B in samples.
Matrix Recovery range Average
serum(n=5) 90-105% 97%
EDTA plasma(n=5) 88-103% 95%
Heparin plasma(n=5) 92-106% 99%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of C4B and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 85-92% 79-96% 95-102% 87-98%
EDTA plasma(n=5) 89-97% 85-94% 87-96% 85-97%
Heparin plasma(n=5) 85-94% 87-96% 92-101% 82-98%