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Rat PPAR-γ(Peroxisome Proliferator Activated Receptor Gamma) ELISA Kit
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ELK1632
Size： Price：
48 T $320.00
96 T $458.00
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Overview

Product name: Rat PPAR-γ(Peroxisome Proliferator Activated Receptor Gamma) ELISA Kit
Reactivity: Rat
Alternative Names: PPAR-G; PPARG1; PPARG2; NR1C3; Glitazone Receptor; Nuclear Receptor Subfamily 1 Group C Member 3; PPARg; Peroxisome Proliferator Activated Receptor Gamma
Assay Type: Sandwich
Sensitivity: 0.059 ng/mL
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length: 3.5h
Research Area: Metabolic pathway;Endocrinology;Cardiovascular biology;Developmental science;Bone metabolism;
Uniprot ID: O88275
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat PPAR-γ. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat PPAR-γ. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat PPAR-γ, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat PPAR-γ in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
10.00 2.126 2.041
5.00 1.628 1.543
2.50 1.128 1.043
1.25 0.847 0.762
0.63 0.527 0.442
0.32 0.335 0.250
0.16 0.179 0.094
0.00 0.085 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant PPAR-γ and the recovery rates were calculated by comparing the measured value to the expected amount of PPAR-γ in samples.
Matrix Recovery range Average
serum(n=5) 77-95% 86%
EDTA plasma(n=5) 80-97% 88%
Heparin plasma(n=5) 82-94% 88%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PPAR-γ and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 86-98% 91-103% 85-97% 87-96%
EDTA plasma(n=5) 87-91% 85-93% 89-97% 85-93%
Heparin plasma(n=5) 89-97% 85-94% 87-96% 85-97%