Products

Human LAB7-2(B-Lymphocyte Activation Antigen B7-2) Microsample ELISA Kit
One-click to copy product information
ELK1606MS
Size： Price：
48 T $320.00
96 T $458.00
96 T*5 $1947.00
Manual Send email to us

Overview

Product name: Human LAB7-2(B-Lymphocyte Activation Antigen B7-2) Microsample ELISA Kit
Reactivity: Human
Alternative Names: CD86; B7-2; B70; FUN-1; CD28LG2; CD28 Antigen Ligand 2,B7-2 Antigen; CTLA-4 counter-receptor B7.2
Assay Type: Sandwich
Sensitivity: 0.056 ng/mL
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: Tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area: Signal transduction;CD & Adhesion molecule;Tumor immunity;Infection immunity;Immune molecule;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human LAB7-2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human LAB7-2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human LAB7-2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human LAB7-2 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
10.00 2.138 2.041
5.00 1.603 1.506
2.50 1.288 1.191
1.25 0.887 0.790
0.63 0.465 0.368
0.31 0.335 0.238
0.16 0.240 0.143
0.00 0.097 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant LAB7-2 and the recovery rates were calculated by comparing the measured value to the expected amount of LAB7-2 in samples.
Matrix Recovery range Average
serum(n=5) 89-117% 103%
EDTA plasma(n=5) 85-106% 96%
Heparin plasma(n=5) 85-94% 90%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of LAB7-2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 88-97% 86-118% 90-118% 85-103%
EDTA plasma(n=5) 81-88% 91-104% 82-101% 90-96%
Heparin plasma(n=5) 93-120% 82-118% 83-97% 94-102%

Product Components