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Mouse NF-κB p105(Nuclear factor NF-kappa-B p105 subunit) Microsample ELISA Kit
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ELK1337MS
Size： Price：
48 T $265.00
96 T $376.00
96 T*5 $1598.00
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Overview

Product name: Mouse NF-κB p105(Nuclear factor NF-kappa-B p105 subunit) Microsample ELISA Kit
Reactivity: Mouse
Alternative Names: NF-KB1; EBP-1; KBF1; NF-Kappa-B; NFKB-P105; NFKB-P50; Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells 1; Nuclear factor NF-kappa-B p105 subunit; NFkB; Nuclear Factor Kappa B
Assay Type: Sandwich
Sensitivity: 0.06 ng/mL
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area: Signal transduction;
Uniprot ID: P25799
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse NF-κBp105. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse NF-κBp105. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse NF-κBp105, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse NF-κBp105 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
10.00 1.783 1.679
5.00 1.332 1.228
2.50 0.907 0.803
1.25 0.636 0.532
0.63 0.502 0.398
0.31 0.369 0.265
0.16 0.218 0.114
0.00 0.104 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant NF-κBp105 and the recovery rates were calculated by comparing the measured value to the expected amount of NF-κBp105 in samples.
Matrix Recovery range Average
serum(n=5) 82-106% 94%
EDTA plasma(n=5) 82-125% 104%
Heparin plasma(n=5) 82-111% 97%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NF-κBp105 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 86-119% 83-92% 93-106% 88-119%
EDTA plasma(n=5) 95-109% 81-98% 95-107% 92-117%
Heparin plasma(n=5) 91-96% 88-107% 84-119% 83-94%

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