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Mouse GROa/CXCL1(Growth Regulated Oncogene Alpha) ELISA Kit
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ELK1238
Size： Price：
48 T $265.00
96 T $376.00
96 T*5 $1598.00
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Product name: Mouse GROa/CXCL1(Growth Regulated Oncogene Alpha) ELISA Kit
Reactivity: Mouse
Alternative Names: CXCL1; GRO1; GROa; GRO-A; MGSA-A; NAP3; SCYB1; Chemokine C-X-C-Motif Ligand 1; Melanoma Growth Stimulating Activity Alpha; Fibroblast Secretory Protein; Neutrophil Activating Protein 3
Assay Type: Sandwich
Sensitivity: 6 pg/mL
Standard: 1000 pg/mL
Detection Range: 15.63-1000 pg/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area: Cytokine;Tumor immunity;Infection immunity;
Uniprot ID: P12850
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse GROa/CXCL1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse GROa/CXCL1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse GROa/CXCL1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse GROa/CXCL1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
1000.00 2.532 2.429
500.00 1.617 1.514
250.00 0.924 0.821
125.00 0.611 0.508
62.50 0.476 0.373
31.25 0.315 0.212
15.63 0.241 0.138
0.00 0.103 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant GROa/CXCL1 and the recovery rates were calculated by comparing the measured value to the expected amount of GROa/CXCL1 in samples.
Matrix Recovery range Average
serum(n=5) 82-95% 88%
EDTA plasma(n=5) 78-96% 87%
Heparin plasma(n=5) 95-107% 101%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of GROa/CXCL1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 79-91% 89-103% 82-95% 86-99%
EDTA plasma(n=5) 87-96% 91-101% 87-98% 89-103%
Heparin plasma(n=5) 86-97% 83-96% 91-103% 96-105%