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Human CA19-9(Carbohydrate Antigen 19-9) Microsample ELISA Kit
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ELK1198MS
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48 T $265.00
96 T $376.00
96 T*5 $1598.00
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Overview

Product name: Human CA19-9(Carbohydrate Antigen 19-9) Microsample ELISA Kit
Reactivity: Human
Alternative Names: sialylated Lewis(a)Antigen; Cancer Antigen 19-9
Assay Type: Sandwich
Sensitivity: 0.28 U/mL
Standard: 50 U/mL
Detection Range: 0.79-50 U/mL
Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Assay Length: 3.5h
Research Area: Tumor immunity;Hepatology;Gastroenterology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CA19-9. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CA19-9. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CA19-9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CA19-9 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (U/mL) OD Corrected OD
50.00 1.898 1.813
25.00 1.537 1.452
12.50 1.041 0.956
6.25 0.790 0.705
3.13 0.544 0.459
1.56 0.345 0.260
0.78 0.225 0.140
0.00 0.085 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant CA19-9 and the recovery rates were calculated by comparing the measured value to the expected amount of CA19-9 in samples.
Matrix Recovery range Average
serum(n=5) 83-90% 87%
EDTA plasma(n=5) 85-127% 106%
Heparin plasma(n=5) 88-114% 101%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CA19-9 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 85-100% 93-112% 92-114% 82-91%
EDTA plasma(n=5) 83-113% 89-102% 87-96% 88-102%
Heparin plasma(n=5) 85-93% 88-117% 95-107% 94-103%

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